The HPRT Rabbit Monoclonal Antibody (CAB8783) is a highly specific antibody designed for research involving the HPRT protein, an enzyme that plays a critical role in the salvage pathway of purine nucleotide biosynthesis. The antibody, generated in rabbits, is validated for use in various applications, including immunohistochemistry and Western blot analysis. It binds specifically to the HPRT protein, allowing for accurate detection and analysis in a wide range of sample types.HPRT is essential for maintaining cellular levels of purine nucleotides, which are necessary for various cellular processes such as DNA and RNA synthesis.
Mutations in the HPRT gene have been linked to Lesch-Nyhan syndrome, a rare genetic disorder characterized by neurological and behavioral abnormalities. Research on HPRT is vital for understanding purine metabolism and its implications in disease development.The HPRT Rabbit Monoclonal Antibody is a valuable tool for studying HPRT expression and function in both normal and diseased cells. Its high specificity and sensitivity make it an ideal choice for researchers investigating purine metabolism, genetic disorders, and potential therapeutic targets related to HPRT activity.
Product Name:
HPRT1 Rabbit Monoclonal Antibody
SKU:
CAB8783
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 119-218 of human HPRT11 (NP_000185.1).
The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.
Purification Method:
Affinity purification
Gene ID:
3251
Clone Number:
ARC1300
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using HPRT11 Rabbit mAb (CAB8783) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
