The HOXA4 Polyclonal Antibody (PAC056836) is a valuable tool for researchers studying the HOXA4 protein, a transcription factor involved in developmental processes and cancer progression. This antibody, produced using rabbits as hosts, exhibits high reactivity with human samples and has been rigorously validated for use in Western blot assays. By specifically targeting the HOXA4 protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in developmental biology and oncology research.HOXA4 plays a pivotal role in regulating gene expression and influencing cellular differentiation, making it a key player in embryonic development and cancer cell behavior.
Understanding the functions and mechanisms of HOXA4 can provide insights into developmental disorders and oncogenesis, paving the way for potential therapeutic interventions targeting this transcription factor. Researchers exploring the roles of HOXA4 in cancer progression, stem cell differentiation, and tissue development will find this antibody indispensable in their studies.
Western Blot. Positive WB detected in: Rat kidney tissue. All lanes: HOXA4 antibody at 2.3µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 35 kDa. Observed band size: 35 kDa.
IHC image of PACO56836 diluted at 1:800 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT-3'.
Synonyms:
Homeobox protein Hox-A4 (Homeobox protein Hox-1.4) (Homeobox protein Hox-1D), HOXA4, HOX1D
UniProt Protein Function:
HOXA4: Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to sites in the 5'-flanking sequence of its coding region with various affinities. The consensus sequences of the high and low affinity binding sites are 5'-TAATGA[CG]-3' and 5'-CTAATTTT- 3'. Belongs to the Antp homeobox family. Deformed subfamily.Protein type: DNA-binding; Transcription factorChromosomal Location of Human Ortholog: 7p15.2Cellular Component: nucleusMolecular Function: sequence-specific DNA binding; transcription factor activityBiological Process: anterior/posterior pattern formation; anatomical structure morphogenesis; regulation of transcription, DNA-dependent; transcription, DNA-dependent; embryonic skeletal morphogenesis
UniProt Protein Details:
NCBI Summary:
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. [provided by RefSeq, Jul 2008]
