The HOXA10 Antibody (CAB8550) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, produced in rabbits, boasts high reactivity with human samples and is validated for use in Western blot applications. By binding specifically to HOXA10, this antibody enables precise detection and analysis in various cell types, making it an essential component for studies in developmental biology and cancer research.HOXA10 is a critical transcription factor that controls the expression of genes involved in cell growth, differentiation, and organ development.
This antibody is validated for use in WB, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.
Product Name:
HOXA10 Antibody
SKU:
CAB8550
Size:
20μL, 100μL
Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Immunogen:
Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:
PL, HOX1, HOX1H, HOX1.8, HOXA10
Positive Sample:
SKOV3, HT-29, Rat kidney, Mouse kidney
Cellular Localization:
Nucleus.
Calculated MW:
42kDa
Observed MW:
53kDa
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. This gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor that may regulate gene expression, morphogenesis, and differentiation. More specifically, it may function in fertility, embryo viability, and regulation of hematopoietic lineage commitment. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the downstream homeobox A9 (HOXA9) gene.
Purification Method
Affinity purification
Gene ID
3206
RRID
AB_2769826
Buffer Information
Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Western blot analysis of various lysates using HOXA10 Rabbit pAb (CAB8550) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 10s.
Western blot analysis of lysates from Mouse kidney, using HOXA10 Rabbit pAb (CAB8550) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (AbGn00021). Exposure time: 10s.
