Anti-HOPX Antibody (CAB15537)
- SKU:
- CAB15537
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
Anti-HOPX Antibody (CAB15537)
The Hopx Polyclonal Antibody (CAB15537) is a valuable tool for researchers studying the Hopx protein, a critical regulator of cardiac development and homeostasis. This antibody, generated in rabbits, exhibits high specificity and sensitivity for detecting Hopx in human samples, making it ideal for use in Western blot applications.Hopx, also known as homeodomain-only protein, has been shown to play a pivotal role in maintaining cardiac function by regulating gene expression and cellular differentiation. Dysregulation of Hopx has been implicated in various cardiovascular diseases, including heart failure and arrhythmias. By targeting the Hopx protein, researchers can gain insights into the underlying mechanisms of cardiac pathophysiology and potentially identify novel therapeutic targets for heart-related disorders.
Furthermore, the Hopx Polyclonal Antibody offers versatility in its application, allowing for the detection and analysis of Hopx in diverse cell types and experimental settings. Its reliability and reproducibility make it a valuable asset for studies in cardiovascular biology, developmental biology, and regenerative medicine. By using this antibody, researchers can advance their understanding of Hopx function and its implications for heart health and disease.
| Antibody Name: | Anti-HOPX Antibody |
| Antibody SKU: | CAB15537 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-73 of human HOPX (NP_631957.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | mouse brain, mouse lung, rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-73 of human HOPX (NP_631957.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSAE TASG PTED QVEI LEYN FNKV DKHP DSTT LCLI AAEA GLSE EETQ KWFK QRLA KWRR SEGL PSEC RSVT D |
| Gene ID: | 84525 |
| Uniprot: | Q9BPY8 |
| Cellular Location: | Nucleus |
| Calculated MW: | 8kDa/10kDa/12kDa |
| Observed MW: | 13kDa |
| Synonyms: | HOPX, CAMEO, HOD, HOP, LAGY, NECC1, OB1, SMAP31, TOTO |
| Background: | The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene. |
| UniProt Protein Function: | HOPX: Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy. May act as a tumor suppressor. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oncoprotein; DNA-binding Chromosomal Location of Human Ortholog: 4q12 Cellular Component: nucleus Molecular Function:protein binding Biological Process: negative regulation of cell differentiation; regulation of transcription from RNA polymerase II promoter; trophectodermal cell differentiation |
| NCBI Summary: | The protein encoded by this gene is a homeodomain protein that lacks certain conserved residues required for DNA binding. It was reported that choriocarcinoma cell lines and tissues failed to express this gene, which suggested the possible involvement of this gene in malignant conversion of placental trophoblasts. Studies in mice suggest that this protein may interact with serum response factor (SRF) and modulate SRF-dependent cardiac-specific gene expression and cardiac development. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Feb 2009] |
| UniProt Code: | Q9BPY8 |
| NCBI GenInfo Identifier: | 60392394 |
| NCBI Gene ID: | 84525 |
| NCBI Accession: | Q9BPY8.1 |
| UniProt Secondary Accession: | Q9BPY8,Q8N0V6, Q96CI1, A8K0Z2, E9PB55, G3V294, |
| UniProt Related Accession: | Q9BPY8 |
| Molecular Weight: | 12,625 Da |
| NCBI Full Name: | Homeodomain-only protein |
| NCBI Synonym Full Names: | HOP homeobox |
| NCBI Official Symbol: | HOPXÂ Â |
| NCBI Official Synonym Symbols: | HOD; HOP; OB1; LAGY; TOTO; CAMEO; NECC1; SMAP31Â Â |
| NCBI Protein Information: | homeodomain-only protein |
| UniProt Protein Name: | Homeodomain-only protein |
| UniProt Synonym Protein Names: | Lung cancer-associated Y protein; Not expressed in choriocarcinoma protein 1; Odd homeobox protein 1 |
| Protein Family: | Homeodomain-only protein |
| UniProt Gene Name: | HOPXÂ Â |
| UniProt Entry Name: | HOP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HOPX antibody (CAB15537) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using HOPX antibody (CAB15537) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using HOPX antibody (CAB15537) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using HOPX antibody (CAB15537) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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