The Hook1 Polyclonal Antibody (CAB4731) is a valuable tool for researchers studying the protein Hook1, which plays a crucial role in intracellular transport and organization of organelles within the cell. This polyclonal antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Hook1 is a key player in the assembly and function of the Golgi apparatus and is essential for proper cellular function and development. By targeting and detecting Hook1 protein expression, researchers can gain insights into its role in various cellular processes, making this antibody ideal for studies in cell biology and neuroscience.
The understanding of Hook1 function is crucial for unraveling its involvement in diseases such as neurodegenerative disorders and cancer, where dysregulation of intracellular transport mechanisms can play a significant role. The Hook1 Polyclonal Antibody provides researchers with a reliable tool to explore the functions of Hook1 and its implications in health and disease.
Product Name:
HOOK1 Rabbit Polyclonal Antibody
SKU:
CAB4731
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 489-728 of human HOOK1 (NP_056972.1).
This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined.
Purification Method:
Affinity purification
Gene ID:
51361
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HOOK1 antibody (CAB4731) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 5s.
