The HNRNPF Polyclonal Antibody is a valuable tool for researchers studying the HNRNPF protein, a key regulator of RNA metabolism and gene expression. This antibody, produced in rabbits, exhibits high specificity for human samples and is validated for use in Western blotting applications.HNRNPF is a multifunctional protein involved in various aspects of RNA processing, including splicing, alternative polyadenylation, and mRNA stability. Its dysregulation has been implicated in a wide range of diseases, including cancer, neurodegenerative disorders, and autoimmune conditions.
By targeting the HNRNPF protein, this antibody enables researchers to investigate its role in diverse cellular processes and disease states. Its versatility and reliability make it an essential tool for studies in molecular biology, genetics, and biomedicine. Explore the function of HNRNPF and uncover its potential as a therapeutic target with the HNRNPF Polyclonal Antibody.
Product Name:
HNRNPF Polyclonal Antibody
SKU:
CAB22872
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant protein of human HNRNPF.
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
HNRPF; mcs94-1; OK/SW-cl.23; HNRNPF
Positive Sample:
HeLa,NIH/3T3
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleoplasm.
Calculated MW:
46kDa
Observed MW:
46kDa
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Purification Method:
Affinity purification
Gene ID:
3185
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates, using HNRNPF Rabbit pAb (CAB22872) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
