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hnRNP E1/PCBP1 Polyclonal Antibody (CAB21733)

SKU:
CAB21733
Product Type:
Antibody
Antibody Type:
Polyclonal Antibody
Reactivity:
Human
Mouse
Rat
Host Species:
rabbit
Isotype:
IgG
Applications:
WB
IHC
$167 - $635
Frequently bought together:

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:hnRNP E1/PCBP1 Polyclonal Antibody
SKU:CAB21733
Size:20uL, 100uL
Isotype:IgG
Host Species:rabbit
Reactivity:Human,Mouse,Rat
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human hnRNP E1/PCBP1 (NP_006187.2).
Sequence:LRLV VPAT QCGS LIGK GGCK IKEI REST GAQV QVAG DMLP NSTE RAIT IAGV PQSV TECV KQIC LVML ETLS QSPQ GRVM TIPY QPMP ASSP VICA GGQD R
Tested Applications:WB IHC-P ELISA
Recommended Dilution:WB,1:500 - 1:1000 IHC-P,1:50 - 1:200
Synonyms:HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1; P1
Positive Sample:HepG2,NIH/3T3,Rat pancreas,HeLa
Conjugate:Unconjugated
Cellular Localization:Cytoplasm, Nucleus.
Calculated MW:37kDa
Observed MW:40kDa

This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.

Purification Method:Affinity purification
Gene ID:5093
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Product ImageWestern blot analysis of various lysates using [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (CAB21733) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.

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