The HNRNP E1/PCBP1 Polyclonal Antibody is a valuable tool for researchers studying RNA binding proteins involved in gene regulation and mRNA processing. This antibody, produced in rabbits, is highly specific and reacts with human samples, making it ideal for use in Western blot applications. By targeting the HNRNP E1/PCBP1 protein, researchers can analyze its expression and function in various cell types, providing insights into its role in cellular processes such as RNA splicing and stability.
HNRNP E1/PCBP1, a multifunctional RNA binding protein, is known to play a crucial role in modulating gene expression and post-transcriptional regulation. Its involvement in mRNA metabolism makes it a compelling target for studies related to cancer, neurodegenerative diseases, and viral infections. By investigating the functions of HNRNP E1/PCBP1, researchers can gain a deeper understanding of its impact on cellular processes and potential implications for disease development and progression.
Product Name:
hnRNP E1/PCBP1 Polyclonal Antibody
SKU:
CAB21733
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human hnRNP E1/PCBP1 (NP_006187.2).
This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.
Purification Method:
Affinity purification
Gene ID:
5093
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] hnRNP E1/PCBP1 Rabbit pAb (CAB21733) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
