The HNRNP A1 Rabbit Monoclonal Antibody is a versatile tool for researchers studying the HNRNP A1 protein, a key player in RNA processing and gene regulation. This high-quality antibody, produced in rabbits, is specifically designed for use in Western blot applications and is highly reactive with human samples.HNRNP A1, also known as heterogeneous nuclear ribonucleoprotein A1, is involved in various cellular processes, including pre-mRNA splicing, mRNA stability, and translation. Dysregulation of HNRNP A1 has been linked to several diseases, including cancer and neurodegenerative disorders, making it an important target for research in these areas.
By using the HNRNP A1 Rabbit Monoclonal Antibody, researchers can accurately detect and analyze HNRNP A1 protein levels in a variety of cell types, helping to further our understanding of its role in disease development and progression. This antibody is a valuable tool for studies in molecular biology, cancer research, and neuroscience, providing valuable insights into the complex mechanisms of gene expression regulation.
Product Name:
hnRNP A1 Rabbit Monoclonal Antibody
SKU:
CAB11564
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human hnRNP A1 (P09651).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
UP 1; ALS19; ALS20; HNRPA1; IBMPFD3; HNRPA1L3; hnRNP A1; hnRNP-A1
Positive Sample:
HeLa,293T,HepG2,Mouse brain,Rat brain
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus.
Calculated MW:
39kDa
Observed MW:
30kDa/34kDa/39kDa
This gene encodes a member of a family of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs), which are RNA-binding proteins that associate with pre-mRNAs in the nucleus and influence pre-mRNA processing, as well as other aspects of mRNA metabolism and transport. The protein encoded by this gene is one of the most abundant core proteins of hnRNP complexes and plays a key role in the regulation of alternative splicing. Mutations in this gene have been observed in individuals with amyotrophic lateral sclerosis 20. Multiple alternatively spliced transcript variants have been found. There are numerous pseudogenes of this gene distributed throughout the genome.hnRNP A1 has three isoforms with MW 30 kDa, 34 kDa and 39 kDa.
Purification Method:
Affinity purification
Gene ID:
3178
Clone Number:
ARC0633
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of various lysates using hnRNP A1 Rabbit mAb (CAB11564) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
