The HNMT Polyclonal Antibody (CAB13499) is a valuable tool for researchers studying histamine N-methyltransferase (HNMT), an enzyme involved in the metabolism of histamine. This antibody, produced in rabbits, is highly specific to human HNMT and is validated for Western blot applications. By binding to the HNMT protein, this antibody enables the detection and analysis of HNMT in various cell types, making it an essential component for studies in allergy research, histamine metabolism, and neurological disorders.HNMT plays a crucial role in histamine degradation, regulating the levels of this important biogenic amine in the body. Dysregulation of HNMT function has been linked to various conditions, including allergies, asthma, and neurological disorders.
Understanding the activity and expression of HNMT is essential for developing targeted therapies and interventions aimed at modulating histamine levels and alleviating symptoms associated with histamine dysregulation.Researchers in the fields of immunology, allergy, and neuroscience will find the HNMT Polyclonal Antibody (CAB13499) to be a valuable tool for investigating the role of HNMT in health and disease. Its specificity, sensitivity, and reliability make it an essential reagent for studies aiming to unravel the complexities of histamine metabolism and its impact on various physiological processes.
Product Name:
HNMT Rabbit Polyclonal Antibody
SKU:
CAB13499
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human HNMT (NP_008826.1).
In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. This gene encodes the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells. Multiple alternatively spliced transcript variants that encode different proteins have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3176
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using HNMT Rabbit pAb (CAB13499) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
