The HNF4G Polyclonal Antibody (CAB16051) is a key tool for researchers studying the HNF4G protein, a transcription factor involved in regulating gene expression in various tissues, including the liver and pancreas. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By targeting the HNF4G protein, researchers can detect and analyze its expression in different cell types, making it invaluable for studies in molecular biology and metabolic research.HNF4G, a member of the hepatocyte nuclear factor family, plays a critical role in controlling the expression of genes involved in glucose and lipid metabolism, as well as cell differentiation and proliferation.
Its dysregulation has been linked to diseases such as diabetes, liver dysfunction, and certain cancers, making it a promising therapeutic target for drug development and personalized medicine. Understanding the function of HNF4G is essential for unveiling its role in metabolic pathways and disease progression, paving the way for innovative treatment strategies.
Product Name:
HNF4G Rabbit Polyclonal Antibody
SKU:
CAB16051
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 366-445 of human HNF4G (NP_004124.4?).
Sequence:
ASND GSHL HHPM HPHL SQDP LTGQ TILL GPMS TLVH ADQI STPE TPLP SPPQ GSGQ EQYK IAAN QASV ISHQ HLSK QKQL
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:1000 - 1:5000 IF/ICC,1:50 - 1:100
Synonyms:
NR2A2; NR2A3; HNF4G
Positive Sample:
Mouse large intestine,Rat liver
Conjugate:
Unconjugated
Cellular Localization:
Nucleus
Calculated MW:
46kDa
Observed MW:
46kDa/50kDa
Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in positive regulation of transcription by RNA polymerase II. Located in several cellular components, including intercellular bridge; mitotic spindle; and nucleoplasm.
Purification Method:
Affinity purification
Gene ID:
3174
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of Mouse large intestine, using HNF4G antibody (CAB16051) at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
