The HNF4A Polyclonal Antibody (CAB2085) is designed for research involving HNF4A, a transcription factor that plays a critical role in regulating gene expression in the liver, pancreas, and other tissues. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in various applications, including Western blot and immunohistochemistry.HNF4A is a key regulator of glucose and lipid metabolism, as well as cell differentiation and proliferation. Dysregulation of HNF4A has been linked to various metabolic disorders, including diabetes and liver diseases.
Therefore, studying the expression and activity of HNF4A is essential for understanding the molecular mechanisms underlying these conditions and developing potential therapeutic interventions.The HNF4A Polyclonal Antibody binds specifically to the HNF4A protein, allowing for precise detection and analysis in different cell types and tissues. Its high sensitivity and specificity make it an indispensable tool for researchers in the fields of hepatology, endocrinology, and metabolic diseases. Uncover the role of HNF4A in health and disease with this reliable antibody.
Product Name:
HNF4A Rabbit Polyclonal Antibody
SKU:
CAB2085
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 226-325 of human HNF4A (NP_849180.1).
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms.
Purification Method:
Affinity purification
Gene ID:
3172
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of SW480 cells, using HNF4A antibody (CAB2085) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
