The HNF4A Polyclonal Antibody (CAB11496) is a valuable tool for researchers studying HNF4A, a transcription factor that plays a crucial role in regulating gene expression in liver and pancreatic cells. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting applications. By targeting the HNF4A protein, researchers can accurately detect and analyze its presence in various cell types, making it ideal for investigations in liver and pancreatic function, as well as diabetes research.
HNF4A, also known as Hepatocyte Nuclear Factor 4 Alpha, is a key player in maintaining liver function, glucose metabolism, and lipid homeostasis. Dysregulation of HNF4A has been linked to various liver diseases, diabetes, and metabolic disorders, making it a promising therapeutic target for drug development. Understanding the role of HNF4A in gene regulation is essential for unraveling its impact on disease pathology and identifying potential treatment strategies.
Product Name:
HNF4a Rabbit Polyclonal Antibody
SKU:
CAB11496
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HNF4a (NP_000448.3).
The protein encoded by this gene is a transcription factor involved in the development of the pancreas, liver, kidney, and intestines. The encoded protein also functions to maintain glucose homeostasis. Several transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
15378
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse liver, using HNF4a antibody (CAB11496) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
