The HNF1A Polyclonal Antibody (CAB3092) is a valuable tool for researchers studying the HNF1A protein, a transcription factor involved in regulating gene expression in the liver, pancreas, and other tissues. This antibody, produced in rabbits, shows high specificity and reactivity with human samples, making it suitable for use in Western blotting and other applications that require detection of HNF1A.HNF1A is known for its role in controlling various biological processes, including glucose metabolism, lipid homeostasis, and cell differentiation.
Dysregulation of HNF1A has been linked to metabolic disorders, such as diabetes and liver diseases, making it a promising target for therapeutic interventions. By using the HNF1A Polyclonal Antibody, researchers can gain insights into the function and regulation of this important transcription factor in health and disease contexts.
Product Name:
HNF1A Rabbit Polyclonal Antibody
SKU:
CAB3092
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-130 of human HNF1α (NP_000536.6).
The protein encoded by this gene is a transcription factor required for the expression of several liver-specific genes. The encoded protein functions as a homodimer and binds to the inverted palindrome 5'-GTTAATNATTAAC-3'. Defects in this gene are a cause of maturity onset diabetes of the young type 3 (MODY3) and also can result in the appearance of hepatic adenomas. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
6927
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HNF1α antibody (CAB3092) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
