The Heme Oxygenase-2 Polyclonal Antibody (CAB2745) is a valuable tool for researchers studying heme oxygenase-2 (HO-2), an essential enzyme involved in heme metabolism and oxidative stress response. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is suitable for use in Western blot applications. By specifically binding to the HO-2 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for investigations in the fields of biochemistry, physiology, and pathology.
HO-2 plays a crucial role in maintaining cellular homeostasis by catalyzing the breakdown of heme into biliverdin, carbon monoxide, and free iron. Dysregulation of HO-2 has been associated with various diseases, including cardiovascular disorders, neurodegenerative conditions, and cancer. By understanding the function and regulation of HO-2, researchers aim to develop targeted therapies that manipulate heme metabolism and oxidative stress responses for the treatment of these diseases.
Product Name:
HMOX2 Rabbit Polyclonal Antibody
SKU:
CAB2745
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human HMOX2 (NP_002125.3).
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. Several alternatively spliced transcript variants encoding three different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3163
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HMOX2 Rabbit pAb (CAB2745) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
