The HMOX1 Polyclonal Antibody (CAB11919) is a valuable tool for researchers studying the heme oxygenase-1 (HMOX1) protein, a key enzyme involved in the breakdown of heme into biliverdin, carbon monoxide, and iron. This polyclonal antibody, generated in rabbits, has high reactivity with human samples and is recommended for use in Western blot applications.HMOX1 plays a crucial role in cellular protection against oxidative stress and inflammation, making it a significant target for research in various fields, including immunology, cancer biology, and cardiovascular diseases. By detecting and analyzing HMOX1 protein expression in different cell types, researchers can gain insights into its role in disease pathogenesis and develop potential therapeutic strategies to target this pathway.
With its high specificity and sensitivity, the HMOX1 Polyclonal Antibody is a reliable tool for researchers seeking to investigate the function and regulation of HMOX1 in health and disease. Its versatility and ease of use make it an ideal choice for studies aiming to uncover the molecular mechanisms underlying heme metabolism and its impact on cellular homeostasis.
Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
Purification Method:
Affinity purification
Gene ID:
3162
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Heme Oxygenase 1 (HO-1/HMOX1) Rabbit pAb (CAB11919) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
