The HMGCS2 Polyclonal Antibody (CAB14244) is a crucial tool for researchers studying the HMGCS2 protein, an enzyme responsible for catalyzing the first step in the mevalonate pathway. Mevalonate is a key intermediate in the biosynthesis of cholesterol, coenzyme Q, and other important molecules. This polyclonal antibody, produced in rabbits, exhibits high specificity and sensitivity towards human samples, making it ideal for use in Western blot applications. With its ability to bind to the HMGCS2 protein, researchers can accurately detect and analyze this enzyme in a variety of cell types.
Given the essential role of the mevalonate pathway in cellular metabolism and lipid synthesis, studying HMGCS2 is crucial for understanding diseases such as hypercholesterolemia, Alzheimer's disease, and cancer. By using this antibody, researchers can gain valuable insights into the regulatory mechanisms underlying these conditions and potentially uncover new therapeutic targets.
Product Name:
HMGCS2 Rabbit Polyclonal Antibody
SKU:
CAB14244
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 269-508 of human HMGCS2 (NP_005509.1).
The protein encoded by this gene belongs to the HMG-CoA synthase family. It is a mitochondrial enzyme that catalyzes the first reaction of ketogenesis, a metabolic pathway that provides lipid-derived energy for various organs during times of carbohydrate deprivation, such as fasting. Mutations in this gene are associated with HMG-CoA synthase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3158
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HMGCS2 Rabbit pAb (CAB14244) at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
