The HMGCL Polyclonal Antibody (CAB8052) is a valuable tool for researchers studying 3-hydroxy-3-methylglutaryl-CoA lyase (HMGCL), an essential enzyme in the ketogenesis pathway. This antibody, produced in rabbits, demonstrates high reactivity with human samples and is validated for use in Western blot analyses.HMGCL plays a crucial role in breaking down fats for energy production, making it a key target for studying metabolic disorders like ketosis and hypercholesterolemia. By binding to the HMGCL protein, this antibody enables accurate detection and analysis in a variety of cell types, making it ideal for research in metabolism, lipid metabolism disorders, and mitochondrial diseases.
Understanding the function and regulation of HMGCL is vital for developing targeted therapies for metabolic disorders and exploring the potential impact on related conditions like obesity, diabetes, and cardiovascular disease. The HMGCL Polyclonal Antibody provides a reliable tool for researchers seeking to delve deeper into the mechanisms underlying these complex physiological processes.
Product Name:
HMGCL Rabbit Polyclonal Antibody
SKU:
CAB8052
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human HMGCL (NP_000182.2).
The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
3155
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using HMGCL Rabbit pAb (CAB8052) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 15s.
