The HMGB4 Polyclonal Antibody (PAC014516) is a valuable tool for researchers studying HMGB4, a member of the high mobility group box (HMGB) family of proteins known for their roles in DNA bending, transcription regulation, and inflammation. This antibody is generated in rabbits and exhibits high reactivity with human samples, making it suitable for Western blot applications. By specifically binding to HMGB4, researchers can detect and analyze the protein in various cell types, making it ideal for studies in immunology, cancer research, and beyond.
HMGB4 is a nuclear protein involved in chromatin organization and gene regulation, with emerging roles in immunity and disease. Its implication in inflammatory pathways and immune response modulation make it a promising target for research into autoimmune disorders, cancer, and other inflammatory conditions. Understanding the function and regulation of HMGB4 is crucial for developing targeted therapies that manipulate immune responses and manage disease progression in these contexts.
Antibody Name:
HMGB4 Antibody (PACO14516)
Antibody SKU:
PACO14516
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:1000-1:2000, WB:1:200-1:1000
Species Reactivity:
Human
Immunogen:
Fusion protein of human HMGB4
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: 231 cells, human fetal liver tissue, Primary antibody: PACO14516(HMGB4 Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes.
Background:
High mobility group protein B4 is a transcription factor that in humans is encoded by the HMGB4 gene.HMGB4 is strongly and preferentially expressed in the adult mouse testis and weakly in the brain, but not in many other tissues. HMGB4 associates with chromatin, and in transfection assays, in contrast to HMGB1, it acts as a potent transcriptional repressor. During spermatogenesis, HMGB4 is present in the euchromatin of late pachytene spermatocytes and haploid round spermatids, whereas stronger expression is observed during the elongation phase, where it localizes to the basal pole of the nucleus in a manner mutually exclusive with H1FNT (H1T2) localized at the apical pole. HMGB4 basal localization is lost in H1FNT-mutant spermatids, showing that H1FNT provides a positional cue for organizing chromatin domains within the nucleus. These results show that HMGB4 and H1FNT specify distinct chromatin domains at the apical and basal poles of the elongating spermatid nucleus.
Synonyms:
high mobility group box 4
UniProt Protein Function:
HMGB4: Belongs to the HMGB family.
UniProt Protein Details:
Chromosomal Location of Human Ortholog: 1p35.1
Cellular Component: chromosome; nucleus
Molecular Function:DNA binding; chromatin binding
Biological Process: chromatin remodeling; regulation of transcription, DNA-dependent
