The HMGB1 Polyclonal Antibody (CAB2553) is a valuable tool for research involving HMGB1, a nuclear protein with roles in inflammation, immunity, and cancer. This antibody, produced in rabbits, exhibits high reactivity with human samples and is specifically validated for use in applications such as Western blotting. By binding to the HMGB1 protein, researchers can effectively detect and analyze its presence in various cell types, making it a versatile option for studies in immunology and oncology.HMGB1, also known as high mobility group box 1 protein, is a key player in the immune response and can actively regulate inflammation, cell differentiation, and cell death.
Its involvement in various disease states, including cancer and inflammatory disorders, underscores its importance as a target for further investigation. By understanding the functions and mechanisms of HMGB1, researchers can potentially develop new therapeutic strategies for treating conditions characterized by dysregulated immune responses.
Product Name:
[KO Validated] HMGB1 Rabbit Polyclonal Antibody
SKU:
CAB2553
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 69-168 of human HMGB1 (NP_002119.1).
This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein.
Purification Method:
Affinity purification
Gene ID:
3146
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using HMGB1 Rabbit pAb (CAB2553) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
