The HMGA1 Rabbit Monoclonal Antibody (CAB4343) is a powerful tool for research involving the HMGA1 protein, a key player in gene regulation and cell growth. This antibody, generated in rabbits, exhibits high specificity and sensitivity for human samples, making it an excellent choice for various experimental applications.HMGA1 is a chromatin-associated protein that interacts with DNA and transcription factors to modulate gene expression. Dysregulation of HMGA1 has been linked to cancer development and progression, making it a promising target for therapeutic intervention.
The HMGA1 Rabbit Monoclonal Antibody enables precise detection and analysis of HMGA1 levels in diverse cell types, facilitating studies in cancer biology and beyond.By using this antibody in Western blot experiments, researchers can gain valuable insights into the role of HMGA1 in cellular processes and disease pathways. Whether investigating the mechanisms of tumorigenesis or exploring novel therapeutic strategies, this antibody provides a reliable tool for advancing scientific knowledge and driving towards new discoveries in the field of molecular biology.
Product Name:
HMGA1 Rabbit Monoclonal Antibody
SKU:
CAB4343
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-107 of human HMGA1 (P17096).
WB,1:500 - 1:2000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 ChIP,3μg antibody for 10μg-15μg of ChromatinChIP-seq,1:50 - 1:100
Synonyms:
HMG-R; HMGIY; HMGA1A; HMGA1
Positive Sample:
HeLa,HepG2,BxPC-3,Raji,Mouse testis
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
12kDa
Observed MW:
18kDa
This gene encodes a chromatin-associated protein involved in the regulation of gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of AT-rich regions in double-stranded DNA. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been identified on multiple chromosomes.
Purification Method:
Affinity purification
Gene ID:
3159
Clone Number:
ARC1060
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Chromatin immunoprecipitation was performed with 25 μg of cross-linked chromatin from HepG2 cells using 5 μg of HMGA1 Rabbit mAb (CAB4343). DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2 (RK20228). The ChIP sequencing results indicate the enrichment pattern of HMGA1 across chromosome 12(upper panel) and the genomic region encompassing HNF1A, a representative gene enriched in HMGA1 (lower panel).
