The HMBS Rabbit Monoclonal Antibody is a highly specific antibody designed for research involving HMBS, a key enzyme in heme biosynthesis. This antibody, developed from rabbit monoclonal cells, exhibits high reactivity with human samples and is validated for use in various applications such as Western blot and immunohistochemistry.HMBS, also known as hydroxymethylbilane synthase, plays a crucial role in the production of heme, a vital component of hemoglobin and other hemoproteins. Dysregulation of HMBS activity has been implicated in various diseases including acute intermittent porphyria.
Therefore, studying the expression and function of HMBS using this antibody is essential for gaining insights into heme biosynthesis and its related disorders.This HMBS Rabbit Monoclonal Antibody offers researchers a reliable tool for investigating the role of HMBS in both physiological and pathological conditions. Its high specificity and sensitivity make it an ideal choice for studies in biochemistry, molecular biology, and clinical research related to heme metabolism and porphyria.
Product Name:
HMBS Rabbit Monoclonal Antibody
SKU:
CAB11701
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 250-350 of human HMBS (P08397).
This gene encodes a member of the hydroxymethylbilane synthase superfamily. The encoded protein is the third enzyme of the heme biosynthetic pathway and catalyzes the head to tail condensation of four porphobilinogen molecules into the linear hydroxymethylbilane. Mutations in this gene are associated with the autosomal dominant disease acute intermittent porphyria. Alternatively spliced transcript variants encoding different isoforms have been described.
Purification Method:
Affinity purification
Gene ID:
3145
Clone Number:
ARC0685
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HMBS Rabbit mAb (CAB11701) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
