The HLA-DRB3 Polyclonal Antibody (CAB12444) is a valuable tool for researchers studying human leukocyte antigen (HLA) DRB3, a key component of the immune system involved in antigen presentation. This polyclonal antibody, generated in rabbits, is highly specific and sensitive for detecting HLA-DRB3 in human samples, making it ideal for use in Western blot applications.HLA-DRB3 is a crucial molecule in the immune response, playing a central role in the recognition and activation of T cells. Research on HLA-DRB3 is essential for understanding immune-mediated diseases and developing targeted therapies.
The HLA-DRB3 Polyclonal Antibody allows for precise detection and analysis of HLA-DRB3 in various cell types, providing valuable insights into immune responses and potential therapeutic interventions.Overall, the HLA-DRB3 Polyclonal Antibody (CAB12444) is a reliable reagent for investigating the role of HLA-DRB3 in immunology, autoimmunity, and transplant rejection. Its high specificity and sensitivity make it a valuable asset for researchers seeking to advance our knowledge of the immune system and develop novel treatment strategies.
Product Name:
HLA-DRB3 Rabbit Polyclonal Antibody
SKU:
CAB12444
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-227 of human HLA-DRB3 (NP_072049.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
Calculated MW:
30kDa
Observed MW:
30kDa
HLA-DRB3 belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DRA) and a beta (DRB) chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. There are multiple pseudogenes of this gene.
Purification Method:
Affinity purification
Gene ID:
3125
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HLA-DRB3 antibody (CAB12444) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
