The HLA-DRB1 Polyclonal Antibody (CAB7685) is a valuable tool for researchers studying the HLA-DRB1 protein, a member of the human leukocyte antigen (HLA) class II molecules involved in immune response regulation. This antibody, generated in rabbits, is highly specific and reactive with human samples, making it ideal for use in various research applications.The HLA-DRB1 antibody is validated for use in Western blotting, immunohistochemistry, and flow cytometry, enabling researchers to detect and analyze the expression of HLA-DRB1 in different cell types and tissues.
By targeting the HLA-DRB1 protein, this antibody allows for the investigation of its role in antigen presentation and immune response modulation, providing insights into immune-related diseases such as autoimmune disorders, infectious diseases, and transplant rejection.With its high specificity and versatility in various experimental techniques, the HLA-DRB1 Polyclonal Antibody (CAB7685) is a valuable tool for researchers aiming to further understand the mechanisms underlying immune response regulation and develop targeted therapies for immune-related disorders.
Product Name:
HLA-DRB1 Rabbit Polyclonal Antibody
SKU:
CAB7685
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human HLA-DRB1 (NP_002115.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
Calculated MW:
30kDa
Observed MW:
30kDa
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells. The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and some alleles have increased frequencies associated with certain diseases or conditions. For example, DRB1*1302 has been related to acute and chronic hepatitis B virus persistence. There are multiple pseudogenes of this gene.
Purification Method:
Affinity purification
Gene ID:
3123
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HLA-DRB1 antibody (CAB7685) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit (AbGn00020)._Exposure time: 30s.
