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HLA-DRB1 Antibody (PACO33872)

SKU:
PACO33872
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Applications:
ELISA
Antibody Type:
Polyclonal Antibody
Conjugation:
Unconjugated
$407
Frequently bought together:

Description

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HLA-DRB1 Antibody (PACO33872)

The HLA-DRB1 Polyclonal Antibody (PACO33872) is a crucial tool for researchers studying the HLA-DRB1 protein, a key player in the immune system's ability to recognize and respond to foreign substances. This antibody, produced in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By binding specifically to the HLA-DRB1 protein, this antibody allows for precise detection and analysis in a variety of cell types, making it ideal for research in immunology, infectious diseases, and transplantation medicine.

HLA-DRB1 is a major histocompatibility complex (MHC) class II protein that presents antigens to T cells, initiating an immune response against pathogens. Variations in the HLA-DRB1 gene have been linked to autoimmune diseases such as rheumatoid arthritis and type 1 diabetes, as well as susceptibility to infectious diseases. The HLA-DRB1 Polyclonal Antibody provides researchers with a powerful tool to investigate the role of HLA-DRB1 in these conditions and develop new therapeutic approaches targeting the immune system.

Antibody Name:HLA-DRB1 Antibody (PACO33872)
Antibody SKU:PACO33872
Size:50ug
Host Species:Rabbit
Tested Applications:ELISA
Recommended Dilutions:
Species Reactivity:Human
Immunogen:Recombinant Human HLA class II histocompatibility antigen, DRB1-3 chain protein (31-266AA)
Form:Liquid
Storage Buffer:Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method:>95%, Protein G purified
Clonality:Polyclonal
Isotype:IgG
Conjugate:Non-conjugated
Background:Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an α and a β chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acid, fication produces increased proteolysis and efficient peptide loading.
Synonyms:HLA class II histocompatibility antigen, DRB1-3 chain (Clone P2-beta-3) (MHC class II antigen DRB1*3), HLA-DRB1
NCBI Summary:HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]
UniProt Code:P01912
NCBI GenInfo Identifier:700576546
NCBI Gene ID:3123
NCBI Accession:CAG28307
UniProt Related Accession:P20039,Q5Y7A7,Q95IE3,Q9GIY3,Q29974,P01912,Q30134,P04229,P01911,Q30167
Molecular Weight:359,205 Da
NCBI Full Name:keyhole limpet hemocyanin1, partial
NCBI Synonym Full Names:major histocompatibility complex, class II, DR beta 1
NCBI Official Symbol:HLA-DRB1  
NCBI Official Synonym Symbols:SS1; DRB1; HLA-DRB; HLA-DR1B  
NCBI Protein Information:major histocompatibility complex, class II, DR beta 1
UniProt Protein Name:Keyhole limpet hemocyanin1
UniProt Synonym Protein Names:Keyhole limpet hemocyanin1
UniProt Gene Name:klh1  

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