The HLA-DRA Rabbit Monoclonal Antibody is a specialized research tool designed for investigating HLA-DRA, a critical component of the human immune system. This high-quality antibody, produced in rabbits, is highly specific and reactive with human samples, making it an essential tool for studying immune responses and antigen presentation. Validated for use in applications such as Western blotting, this antibody binds specifically to the HLA-DRA protein, allowing for precise detection and analysis in a variety of cell types.
HLA-DRA is a key player in the immune response, involved in antigen presentation and activation of T-cells. By targeting HLA-DRA, researchers can gain valuable insights into immune function, autoimmune diseases, and immunotherapy development. This antibody is ideal for studies in immunology, cancer research, and vaccine development, providing a valuable tool for understanding the role of HLA-DRA in health and disease.
Product Name:
HLA-DRA Rabbit Monoclonal Antibody
SKU:
CAB10863
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human HLA-DRA (P01903).
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
Calculated MW:
29kDa
Observed MW:
33kDa
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
Purification Method:
Affinity purification
Gene ID:
3122
Clone Number:
ARC0518
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Raji cells, using HLA-DRA Rabbit mAb (CAB10863) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
