The HLA-DRA Polyclonal Antibody (CAB1579) is designed for research involving the human leukocyte antigen (HLA) protein family, specifically the HLA-DRA molecule. This antibody, raised in rabbits, is highly reactive with human samples and is validated for use in applications such as Western blot and immunohistochemistry.HLA molecules play a crucial role in the immune system, specifically in presenting antigens to T cells to initiate an immune response. The HLA-DRA protein is involved in antigen presentation and is essential for the immune system to recognize and respond to foreign invaders such as viruses and bacteria.
Research involving HLA-DRA is important for understanding immune responses in diseases such as autoimmune disorders, infections, and cancer. By studying the expression and function of HLA-DRA, researchers can gain insight into how the immune system recognizes and targets abnormal cells.Overall, the HLA-DRA Polyclonal Antibody is a valuable tool for researchers investigating the role of HLA molecules in immune responses and diseases, ultimately contributing to the development of new therapies and treatments.
Product Name:
HLA-DRA Rabbit Polyclonal Antibody
SKU:
CAB1579
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-216 of human HLA-DRA (NP_061984.2).
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
Calculated MW:
29kDa
Observed MW:
37kDa
HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. This molecule is expressed on the surface of various antigen presenting cells such as B lymphocytes, dendritic cells, and monocytes/macrophages, and plays a central role in the immune system and response by presenting peptides derived from extracellular proteins, in particular, pathogen-derived peptides to T cells. The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5.
Purification Method:
Affinity purification
Gene ID:
3122
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HLA-DRA Rabbit pAb (CAB1579) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
