The HLA-DQA1 Rabbit Monoclonal Antibody is a powerful tool for research involving the HLA-DQA1 gene, which encodes a protein important for immune response regulation. This antibody, developed using rabbit monoclonal technology, has high affinity and specificity for human samples, making it ideal for experiments in immunology and autoimmune disease research.The HLA-DQA1 protein is a component of the human leukocyte antigen (HLA) complex, which plays a crucial role in presenting antigens to the immune system. By targeting HLA-DQA1, researchers can explore the mechanisms of antigen presentation and immune recognition, shedding light on autoimmune diseases, transplantation immunology, and infectious diseases.
With its superior performance in various applications, including immunofluorescence and flow cytometry, the HLA-DQA1 Rabbit Monoclonal Antibody is a valuable resource for scientists studying the complex interactions of the immune system. Its precision and reliability offer researchers the opportunity to delve deeper into the role of HLA-DQA1 in health and disease, paving the way for innovative therapeutic strategies in the future.
Product Name:
HLA-DQA1 Rabbit Monoclonal Antibody
SKU:
CAB11252
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 155-254 of human HLA-DQA1 (P01909).
Cell membrane, Endoplasmic reticulum membrane, Endosome membrane, Golgi apparatus, Lysosome membrane, Single-pass type I membrane protein, trans-Golgi network membrane.
Calculated MW:
28kDa
Observed MW:
28kDa
HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
Purification Method:
Affinity purification
Gene ID:
3117
Clone Number:
ARC0564
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HLA-DQA1 Rabbit mAb (CAB11252) at 1：1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
