The HLA-DOA Polyclonal Antibody (CAB15277) is a specialized research tool designed for studying the human leukocyte antigen DOA protein. This antibody is generated in rabbits and exhibits high reactivity with human samples, making it perfect for Western blot applications. By binding specifically to the HLA-DOA protein, researchers can effectively detect and analyze its presence in a variety of cell types.HLA-DOA is a key player in immune regulation, particularly in the context of antigen presentation and T-cell activation.
By understanding the function of HLA-DOA, researchers can gain insights into immune responses and potentially develop new treatments for autoimmune diseases, infectious diseases, and cancer. This antibody is a valuable asset for studies in immunology and oncology, providing a reliable tool for investigating the role of HLA-DOA in health and disease.
Product Name:
HLA-DOA Rabbit Polyclonal Antibody
SKU:
CAB15277
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 26-217 of human HLA-DOA (NP_002110.1).
Endosome membrane, Lysosome membrane, Single-pass type I membrane protein.
Calculated MW:
28kDa
Observed MW:
28kDa
HLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level.
Purification Method:
Affinity purification
Gene ID:
3111
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HLA-DOA Rabbit pAb (CAB15277) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90S.
