The HLA-DMA Polyclonal Antibody (CAB6922) is a valuable tool for researchers studying the human leukocyte antigen HLA-DMA, a key protein involved in the immune system's ability to recognize and respond to foreign invaders. This antibody, generated in rabbits, specifically targets the HLA-DMA protein in human samples and is validated for use in Western blot applications.HLA-DMA plays a crucial role in antigen processing and presentation, serving as a vital component in the activation of T cells and the initiation of immune responses. Understanding the function and regulation of HLA-DMA is essential for unraveling the complexities of the immune system and developing therapeutic strategies for conditions such as autoimmune diseases and transplant rejection.
By using the HLA-DMA Polyclonal Antibody, researchers can efficiently detect and analyze HLA-DMA expression in various cell types, providing valuable insights into immune responses and potential therapeutic targets. This antibody is an excellent choice for studies in immunology, transplantation, and autoimmune disease research.
Product Name:
HLA-DMA Rabbit Polyclonal Antibody
SKU:
CAB6922
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-233 of human HLA-DMA (NP_006111.2).
Late endosome membrane, Lysosome membrane, Single-pass type I membrane protein.
Calculated MW:
29kDa
Observed MW:
35kDa
HLA-DMA belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta chain (DMB), both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and the cytoplasmic tail.
Purification Method:
Affinity purification
Gene ID:
3108
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using HLA-DMA antibody (CAB6922) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
