The HLA-C Polyclonal Antibody (CAB1013) is a valuable tool for researchers studying the human leukocyte antigen (HLA) system, specifically HLA-C, a key player in immune response regulation. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for Western blot applications. By binding to the HLA-C protein, this antibody enables accurate detection and analysis in a variety of cell types.HLA-C is involved in presenting foreign antigens to the immune system, playing a crucial role in immune recognition and response. Dysregulation of HLA-C has been linked to autoimmune diseases, viral infections, and other immune-related disorders.
The HLA-C Polyclonal Antibody is a valuable tool for investigating the function of HLA-C in these contexts, shedding light on its role in disease development and potential therapeutic strategies.Overall, the HLA-C Polyclonal Antibody (CAB1013) is a reliable reagent for researchers interested in understanding the role of HLA-C in immune response modulation and its implications for various diseases. Its high sensitivity and specificity make it an essential tool for immunology and disease research.
Product Name:
HLA-C Rabbit Polyclonal Antibody
SKU:
CAB1013
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 25-308 of human HLA-C (P30499).
HLA-C belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. About 6000 HLA-C alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.
Purification Method:
Affinity purification
Gene ID:
3107
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using HLA-A+HLA-B+HLA-C Rabbit pAb (CAB1013) at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
