The HLA-B Polyclonal Antibody (CAB1285) is a powerful tool for researchers studying the HLA-B gene, which encodes for a protein involved in presenting antigens to the immune system. This antibody, generated in rabbits, exhibits high specificity for human samples and has been rigorously validated for use in Western blot experiments.HLA-B is a key player in the immune response, aiding in the identification of infected or abnormal cells by T cells. Its role in antigen presentation makes it a crucial component of the adaptive immune system and a target of interest in immunology research.
By targeting HLA-B with this antibody, researchers can gain insight into the function and regulation of this important immune protein.Furthermore, the HLA-B Polyclonal Antibody is suitable for use in a variety of cell types, allowing for versatile applications in immunological studies and investigations into diseases characterized by immune dysregulation. Its specificity and reliability make it an essential tool for scientists seeking to deepen their understanding of the immune response and explore potential therapeutic interventions related to HLA-B function.
Product Name:
HLA-B Rabbit Polyclonal Antibody
SKU:
CAB1285
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HLA-B (NP_005505.2).
HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.
Purification Method:
Affinity purification
Gene ID:
3106
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using HLA-A+HLA-B+HLA-C Rabbit pAb (CAB1285) at 1:3000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
