The HLA-A Polyclonal Antibody (CAB17494) is a valuable tool for researchers studying the HLA-A protein, a key molecule involved in the immune response. This antibody, produced in rabbits, has high reactivity with human samples and is validated for use in Western blot applications. By specifically targeting the HLA-A protein, this antibody enables precise detection and analysis in various cell types, making it ideal for investigations in immunology and cancer research.HLA-A, also known as human leukocyte antigen-A, is crucial for presenting antigens to T cells and initiating immune responses.
Understanding the function and expression of HLA-A is essential for studying immune recognition and response, as well as for developing targeted therapies for diseases like cancer and autoimmune disorders. The HLA-A Polyclonal Antibody facilitates detailed research into the role of HLA-A in immune regulation and disease progression, providing valuable insights for potential therapeutic interventions.
Product Name:
HLA-A Rabbit Polyclonal Antibody
SKU:
CAB17494
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human HLA-A (NP_002107.3).
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. More than 6000 HLA-A alleles have been described. The HLA system plays an important role in the occurrence and outcome of infectious diseases, including those caused by the malaria parasite, the human immunodeficiency virus (HIV), and the severe acute respiratory syndrome coronavirus (SARS-CoV). The structural spike and the nucleocapsid proteins of the novel coronavirus SARS-CoV-2, which causes coronavirus disease 2019 (COVID-19), are reported to contain multiple Class I epitopes with predicted HLA restrictions. Individual HLA genetic variation may help explain different immune responses to a virus across a population.
Purification Method:
Affinity purification
Gene ID:
3105
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells using HLA-A+HLA-B+HLA-C Rabbit pAb (CAB17494) at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 15s.
