The HK1 Polyclonal Antibody is a vital tool for researchers studying the HK1 protein, a key player in cellular metabolism and energy production. This antibody, developed using high-quality recombinant protein as an immunogen, is highly specific and sensitive for detecting HK1 in human samples. Validated for use in various applications, including Western blot and immunohistochemistry, this antibody allows for precise and accurate analysis of HK1 expression levels in different cell types.HK1, a hexokinase enzyme, is responsible for initiating the first step in glycolysis by catalyzing the phosphorylation of glucose to glucose-6-phosphate. Its role in energy metabolism and regulation makes it a crucial target for research in areas such as cancer, diabetes, and neurodegenerative diseases.
By studying HK1 expression and activity, researchers can gain valuable insights into the metabolic changes associated with these conditions and potentially identify novel therapeutic targets.Overall, the HK1 Polyclonal Antibody is a valuable tool for researchers interested in unraveling the intricate mechanisms of cellular metabolism and its impact on disease development. By enabling precise detection and analysis of HK1, this antibody contributes to advancing our understanding of metabolic pathways and their dysregulation in various pathological states.
Product Name:
HK1 Polyclonal Antibody
SKU:
CAB0546
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human HK1 (NP_000179.2).
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Purification Method:
Affinity purification
Gene ID:
3098
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using HK1 Rabbit pAb (CAB0546).Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.
