The HK1 Rabbit Monoclonal Antibody is a valuable tool for researchers studying the HK1 protein, which is involved in glycolysis and is highly expressed in cancer cells. This antibody, produced using rabbits, has been validated for use in various applications, including Western blot and immunohistochemistry. Its high specificity and sensitivity make it ideal for detecting and analyzing HK1 protein levels in biological samples.HK1 is a key enzyme in the glycolytic pathway and is overexpressed in many cancer types, contributing to the high energy demands of rapidly proliferating tumor cells.
Targeting HK1 has emerged as a promising strategy for cancer therapy, making this antibody essential for investigating the role of HK1 in cancer progression and metabolism.By using the HK1 Rabbit Monoclonal Antibody, researchers can gain valuable insights into the functions of HK1 in cancer biology and explore its potential as a therapeutic target. Its reliable performance and versatility in different experimental techniques make it a valuable asset in studies aimed at understanding the molecular mechanisms underlying cancer development and progression.
Product Name:
[KO Validated] HK1 Rabbit Monoclonal Antibody
SKU:
CAB0533
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Hexokinase 1 (P19367).
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in several transcript variants which encode different isoforms, some of which are tissue-specific.
Purification Method:
Affinity purification
Gene ID:
3098
Clone Number:
ARC0256
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from wild type (WT) and HK1 knockout (KO) 293T cells, using [KO Validated] HK1 Rabbit mAb (CAB0533) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
