The Histone H4 Polyclonal Antibody (CAB17024) is a valuable tool for researchers studying histone H4, a crucial component of chromatin involved in gene regulation and DNA packaging. This antibody, generated in rabbits, exhibits strong reactivity with human samples and has been validated for use in Western blot applications. By targeting the histone H4 protein, researchers can effectively analyze its expression and modifications in various cell types, providing key insights into epigenetic regulation and gene expression dynamics.Histone H4 is essential for maintaining chromatin structure and gene transcription, making it a key player in various biological processes such as cell proliferation, differentiation, and development.
Dysregulation of histone H4 has been implicated in diseases like cancer, neurological disorders, and metabolic conditions, highlighting its potential as a therapeutic target for drug development and disease intervention.With its high specificity and sensitivity in detecting histone H4, the Histone H4 Polyclonal Antibody (CAB17024) is an invaluable resource for researchers delving into epigenetics, chromatin biology, and disease mechanisms. By understanding the role of histone H4 in cellular function and pathology, scientists can pave the way for innovative treatments and interventions in various fields of biomedical research.
Product Name:
Histone H4 Rabbit Polyclonal Antibody
SKU:
CAB17024
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H4 (NP_003529.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Histone H4 Rabbit pAb (CAB17024) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
