The Histone H4 Polyclonal Antibody is a valuable tool for researchers studying histone proteins, which play a crucial role in gene expression and DNA packaging. This antibody, produced in rabbits, is highly specific and sensitive for detecting Histone H4 in human samples. Validated for use in Western blot applications, it allows for the detection and analysis of Histone H4 in various cell types.Histone H4 is a core histone protein that forms the structural unit of nucleosomes, around which DNA is wrapped in the cell nucleus. Modifications to Histone H4, such as acetylation and methylation, play a key role in regulating gene expression and chromatin structure.
Dysregulation of histone proteins has been linked to various diseases, including cancer, making the Histone H4 Polyclonal Antibody a valuable tool for researchers studying epigenetics and chromatin biology.By using the Histone H4 Polyclonal Antibody, researchers can gain insights into the role of histone proteins in gene regulation, chromatin remodeling, and disease development. This antibody is essential for studies in epigenetics, cancer research, and developmental biology, providing a reliable tool for investigating the complex mechanisms of gene expression and DNA packaging.
Product Name:
Histone H4 Polyclonal Antibody
SKU:
CAB21676
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H4 (NP_003529.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using [KO Validated] Histone H4 Rabbit pAb (CAB21676) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
_WB_01__98485.1701341660.jpg?c=2&imbypass=on "Western blot analysis of extracts of various cell lines, using Histone H4 antibody (CAB21676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
