The Histone H4 Polyclonal Antibody (CAB1131) is a valuable tool for research involving histone H4, a core component of nucleosomes that plays a crucial role in DNA packaging and gene regulation. This antibody, generated in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. It specifically recognizes the histone H4 protein, allowing for precise detection and analysis in various cell types.Histone H4 is involved in chromatin structure and function, impacting gene expression and cellular processes such as DNA repair and replication.
Research on histone H4 has implications in epigenetics, cancer biology, and developmental biology. By targeting histone H4 with this antibody, researchers can gain insights into the mechanisms underlying these biological processes and potentially uncover new therapeutic targets for diseases such as cancer.
Product Name:
Histone H4 Rabbit Polyclonal Antibody
SKU:
CAB1131
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H4 (NP_003529.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
Purification Method:
Affinity purification
Gene ID:
8359
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Histone H4 Rabbit pAb (CAB1131) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
