The Acetyl-Histone H3 (K36) Polyclonal Antibody (CAB16077) is a valuable tool for researchers studying epigenetic modifications, specifically acetylation of histone H3 at lysine 36. This antibody, raised in rabbits, is highly specific to acetylated histone H3 at K36 and is validated for use in various applications, including Western blot and immunohistochemistry.Histone acetylation at specific lysine residues plays a critical role in gene regulation and chromatin remodeling, making it an essential focus in the field of epigenetics. Acetylation of histone H3 at K36 has been implicated in various biological processes, including transcriptional activation, DNA replication, and DNA repair.
The Acetyl-Histone H3 (K36) Polyclonal Antibody (CAB16077) enables researchers to specifically detect and analyze acetylation of histone H3 at K36 in various samples, making it an ideal tool for studies in chromatin biology, gene regulation, and cancer research. Understanding the role of histone acetylation at K36 can provide valuable insights into the mechanisms underlying various biological processes and diseases, helping to advance our knowledge and develop potential therapeutic interventions.
Product Name:
Acetyl-Histone H3-K36 Rabbit Polyclonal Antibody
SKU:
CAB16077
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K36 of human Histone H3 (NP_003484.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H3-K36 Rabbit pAb (CAB16077) at 1:1000 dilution.HeLa cells and NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
