The Histone H3 Polyclonal Antibody (CAB2348) is a valuable tool for researchers studying histone proteins and their role in gene regulation and epigenetics. This antibody, raised in rabbits, is highly specific and reacts with histone H3 in a variety of samples, including human tissues. Validated for use in Western blot applications, this antibody binds specifically to histone H3, allowing for precise detection and analysis in a wide range of cell types.Histone H3 is a critical component of chromatin structure and plays a key role in regulating gene expression through modifications such as methylation and acetylation. Dysregulation of histone H3 has been implicated in various diseases, including cancer and neurological disorders, making it an important target for research.
By studying histone H3 using this antibody, researchers can gain insights into the mechanisms underlying these diseases and potentially develop new therapeutic strategies.Overall, the Histone H3 Polyclonal Antibody (CAB2348) is a powerful tool for researchers interested in unraveling the complex roles of histone proteins in gene regulation and disease pathology. Its high specificity and reliability in detecting histone H3 make it a valuable asset in the field of epigenetics and molecular biology.
Product Name:
Histone H3 Rabbit Polyclonal Antibody
SKU:
CAB2348
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 36-136 of human HIST3H3 (NP_003520.1).
WB,1:2000 - 1:10000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,3μg antibody for 5μg-10μg of Chromatin
Synonyms:
H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Histone H3
Positive Sample:
293F,NIH/3T3,PC-12
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
16kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of various lysates using Histone H3 Rabbit pAb (CAB2348) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates / proteins: 25 μg per lane.Blocking buffer: 3 % nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
