Histone H3 Monoclonal Antibody (MACO0012)
- SKU:
- MACO0012
- Product Type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Yeast
- Host Species:
- Mouse
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IF
- IP
- Antibody Type:
- Monoclonal Antibody
- Conjugation:
- Unconjugated
Description
Anti-Histone H3 Antibody (MACO0012)
The Histone H3 Monoclonal Antibody (MACO0012) is a powerful tool for researchers studying histone modifications and epigenetic regulation. Histone H3 is a crucial component of chromatin structure and plays a key role in gene expression and cellular differentiation. This antibody, monoclonal in nature, has been specifically designed to target Histone H3, allowing for precise detection and analysis in a variety of experimental settings.It is well-suited for use in techniques such as Western blotting and immunohistochemistry, and has been validated for its specificity and sensitivity in detecting Histone H3 in human samples.
Its high reactivity and reliability make it an essential tool for studies in the fields of epigenetics, cancer biology, and developmental biology.By using the Histone H3 Monoclonal Antibody (MACO0012), researchers can gain valuable insights into the role of histone modifications in gene regulation, cellular differentiation, and disease development. Its versatility and accuracy make it an indispensable resource for any laboratory conducting research in the field of epigenetics.
| Product Name: | Anti-Histone H3 Antibody |
| Product Sku: | MACO0012 |
| Size: | 50ug |
| Host Species: | Mouse |
| Tested Applications: | ELISA, WB, IF, IP |
| Recommended Dilutions: | WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000 |
| Species Reactivity: | Human,Mouse,Rat,Yeast |
| Immunogen: | Recombinant Protein |
| Form: | Liquid |
| Storage Buffer: | PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol. |
| Purification Method: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Clonality: | Monoclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
| Background: | |
| Synonyms: | HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/ |
 | IF analysis of Hela, diluted at 1:200. |
 | Western blot analysis of 1) Hela, 2) Raw, 3) Mouse Brain Tissue, 4) Rat Brain Tissue, diluted at 1:5000. |
 | 1) Input: Hela Cell Lysate 2) IP product: IP dilute 1:200 |
| UniProt Protein Function: | H3: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Belongs to the histone H3 family. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.2 Cellular Component: extracellular region; membrane; nuclear chromosome; nuclear chromosome, telomeric region; nucleoplasm; nucleosome; nucleus; protein complex Molecular Function:cadherin binding; histone binding; protein binding Biological Process: blood coagulation; cellular protein metabolic process; chromatin silencing at rDNA; DNA replication-dependent nucleosome assembly; establishment and/or maintenance of chromatin architecture; negative regulation of gene expression, epigenetic; nucleosome assembly; positive regulation of gene expression, epigenetic; protein heterotetramerization; RNA-mediated gene silencing; telomere organization and biogenesis |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P68431 |
| NCBI GenInfo Identifier: | 55977055 |
| NCBI Gene ID: | 8357 |
| NCBI Accession: | P68431.2 |
| UniProt Secondary Accession: | P68431,P02295, P02296, P16106, Q6ISV8, Q6NWP8, Q6NWP9 Q6NXU4, Q71DJ3, Q93081, A0PJT7, A5PLR1, |
| UniProt Related Accession: | P68431 |
| Molecular Weight: | 15kDa |
| NCBI Full Name: | Histone H3.1 |
| NCBI Synonym Full Names: | histone cluster 1 H3 family member h |
| NCBI Official Symbol: | HIST1H3HÂ Â |
| NCBI Official Synonym Symbols: | H3/k; H3FK; H3F1KÂ Â |
| NCBI Protein Information: | histone H3.1 |
| UniProt Protein Name: | Histone H3.1 |
| UniProt Synonym Protein Names: | Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l |
| UniProt Gene Name: | HIST1H3AÂ Â |
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