The Anti-Monomethyl H2B (E105) Antibody (CAB20230) is a crucial tool for researchers studying post-translational modifications of histone proteins. This polyclonal antibody, generated in rabbits, specifically targets the monomethylated form of histone H2B at the E105 site. It has been validated for use in various applications, including Western blotting, immunoprecipitation, and immunofluorescence.Histone modifications play a key role in regulating gene expression and chromatin structure, and monomethylation of histone H2B at the E105 site has been linked to transcriptional activation.
By targeting this specific modification, researchers can gain insight into the epigenetic mechanisms underlying gene regulation and cellular function.The Anti-Monomethyl H2B (E105) Antibody is highly sensitive and specific, making it an essential tool for studies in epigenetics, gene expression, and chromatin biology. Its versatility in different experimental techniques makes it a valuable resource for scientists investigating the role of histone modifications in various biological processes.
Product Name:
MonoMethyl-H2B-E105 Rabbit Polyclonal Antibody
SKU:
CAB20230
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
A synthetic monomethylated peptide around E105 of human H2B (NP_066406.1).
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
3018
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using MonoMethyl-H2B-E105 Rabbit pAb (CAB20230) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 180s.
