The Histone H2B Polyclonal Antibody (PACO02175) is a valuable tool for researchers studying histone modifications and chromatin structure. Histone H2B is a core component of the nucleosome, playing a key role in regulating gene expression and DNA packaging. This antibody, produced in rabbits, exhibits high affinity and specificity for histone H2B in human samples, making it an ideal choice for various applications, including Western blot analysis.Histone H2B is known to undergo various post-translational modifications, such as methylation, acetylation, and ubiquitination, which can impact gene expression and chromatin dynamics. By using the PACO02175 antibody, researchers can investigate the role of histone H2B modifications in various cellular processes, including transcriptional regulation, DNA repair, and epigenetic inheritance.
Furthermore, understanding the regulation of histone H2B and its modifications is essential for unraveling the complex mechanisms underlying gene expression and cell differentiation. This antibody provides a reliable tool for studying histone H2B dynamics in the context of development, disease, and epigenetic inheritance. Its versatility and specificity make it an invaluable asset for researchers working in the fields of epigenetics, chromatin biology, and cancer research.
Antibody Name:
Histone H2B Antibody
Antibody SKU:
PACO02175
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
WB:1:500-1:2000
Species Reactivity:
Human, Mouse, Rat
Immunogen:
synthesized peptide derived from the Internal region of human Histone H2B.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
