The Histone H2B Antibody (PACO00912) is a valuable tool for researchers studying histone proteins and their role in epigenetic regulation. Histone H2B is one of the core histones that make up the nucleosome, the basic unit of chromatin structure. This polyclonal antibody, raised in rabbits, is highly specific for human samples and has been validated for use in Western blot applications.Histone H2B plays a crucial role in gene expression and DNA packaging, making it a key player in processes like transcriptional regulation and chromatin remodeling. Dysregulation of histone proteins has been linked to various diseases, including cancer, neurological disorders, and autoimmune conditions.
Understanding the function of histone H2B and its modifications can provide important insights into the mechanisms underlying these diseases.By using the Histone H2B Antibody, researchers can detect and analyze the levels of histone H2B in different cell types and experimental conditions. This antibody is a valuable tool for studying epigenetic changes and their impact on gene expression, paving the way for new discoveries in the fields of molecular biology, genomics, and personalized medicine.
Antibody Name:
Histone H2B Antibody
Antibody SKU:
PACO00912
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC, IF
Recommended Dilutions:
WB:1:500-1:2000, IHC:1:100-1:300, IF:1:200-1:1000
Species Reactivity:
Human, Mouse
Immunogen:
synthesized peptide derived from the N-terminal region of human Histone H2B.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Synonyms:
HIST1H2BH; H2BFJ; Histone H2B type 1-H; Histone H2B.j; H2B/j
UniProt Protein Function:
H2B1H: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.2Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: DNA bindingBiological Process: nucleosome assembly
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015]
