The Hist3H3 Polyclonal Antibody (CAB2352) is specifically designed for research involving Hist3H3, a histone protein that plays a crucial role in gene regulation and chromatin structure. This antibody, generated in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the Hist3H3 protein, this antibody enables precise detection and analysis in various cell types, making it an ideal tool for studies in epigenetics and cancer research.Hist3H3 is a key player in the epigenetic regulation of gene expression, influencing important biological processes such as DNA replication, DNA repair, and transcriptional activation. Dysregulation of Hist3H3 has been implicated in various diseases including cancer, developmental disorders, and neurological conditions.
Investigating the function of Hist3H3 is essential for unraveling the complexities of epigenetic mechanisms and developing targeted therapies for diseases associated with aberrant histone modifications.With its high specificity and sensitivity, the Hist3H3 Polyclonal Antibody offers researchers a reliable tool for exploring the intricate role of Hist3H3 in gene regulation and chromatin remodeling. By understanding the molecular pathways involving Hist3H3, scientists can gain valuable insights into disease mechanisms and potentially identify novel therapeutic targets for improving human health.
Product Name:
Histone H3 Rabbit Polyclonal Antibody
SKU:
CAB2352
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 50-136 of human HIST3H3 (NP_003484.1).
WB,1:2000 - 1:10000 IHC-P,1:50 - 1:200 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cellsChIP,5μg antibody for 5μg-10μg of Chromatin
Synonyms:
H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Histone H3
Positive Sample:
HeLa,HepG2,Raji,Mouse liver,Rat lung
Conjugate:
Unconjugated
Cellular Localization:
Chromosome, Nucleus.
Calculated MW:
16kDa
Observed MW:
17kDa
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Purification Method:
Affinity purification
Gene ID:
8290/8350
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Histone H3 Rabbit pAb (CAB2352) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
