The Hist2H2BE Polyclonal Antibody (CAB1958) is a valuable tool for researchers studying Hist2H2BE, a variant of the H2B histone family. This antibody, raised in rabbits, is highly specific and reactive towards human samples, allowing for reliable detection in Western blot applications. By binding to the Hist2H2BE protein, this antibody enables precise analysis and visualization in various cell types, making it ideal for studies in epigenetics and chromatin research.Hist2H2BE is a histone variant involved in chromatin remodeling and gene regulation, playing a critical role in the organization and compaction of DNA within the nucleus.
Understanding the function and expression of Hist2H2BE is essential for unraveling the complex mechanisms of gene expression and epigenetic regulation. This antibody provides researchers with a powerful tool to investigate the role of Hist2H2BE in normal cellular processes and its potential implications in diseases such as cancer and developmental disorders.
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Purification Method:
Affinity purification
Gene ID:
3017/8349
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using Histone H2B Rabbit pAb (CAB1958) at 1:10000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
