The Acetyl-Histone H2B (K12) Polyclonal Antibody (CAB15619) is a crucial tool for researchers studying epigenetics and gene regulation. This antibody, produced in rabbits, is highly specific to acetylated histone H2B at lysine 12 and is validated for use in applications such as Western blotting and immunohistochemistry.Histone acetylation at specific lysine residues, such as K12 on histone H2B, plays a critical role in regulating gene expression by modulating chromatin structure and accessibility. The Acetyl-Histone H2B (K12) Polyclonal Antibody allows for the detection and analysis of this post-translational modification in various cell types, providing valuable insights into the epigenetic mechanisms underlying cellular processes.
Research on acetylated histone H2B at K12 has implications in understanding gene regulation, development, and diseases such as cancer. By studying the acetylation status of histone H2B, researchers can uncover novel therapeutic targets and potential treatment strategies for various conditions. The high specificity and affinity of this antibody make it an essential tool for investigating the role of histone acetylation in gene expression dynamics.
Product Name:
Acetyl-Histone H2B-K12 Rabbit Polyclonal Antibody
SKU:
CAB15619
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat,Other (Wide Range Predicted)
Immunogen:
A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1).
Sequence:
APKK G
Tested Applications:
WBIHC-PIF/ICCChIPELISA
Recommended Dilution:
WB,1:500 - 1:1000 IHC-P,1:50 - 1:200 IF/ICC,1:50 - 1:200 ChIP,5μg antibody for 5μg-10μg of Chromatin
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity.
Purification Method:
Affinity purification
Gene ID:
3017/8349
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Acetyl-Histone H2B-K12 Rabbit pAb (CAB15619) at 1:1000 dilution.HeLa cells and C2C12 cells and C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
