The HIST1H2BN Polyclonal Antibody (PAC040210) is a valuable tool for researchers studying histone proteins and their role in gene regulation. Histones are crucial for packaging DNA into chromatin and regulating gene expression. This antibody, produced in rabbits, is highly specific to the HIST1H2BN protein and is validated for use in various applications, including Western blotting.HIST1H2BN is a member of the histone H2B family and is involved in chromatin structure and gene transcription. Abnormalities in histone proteins have been linked to various diseases, including cancer and developmental disorders.
By using this antibody, researchers can analyze the expression levels and localization of HIST1H2BN in different cell types, providing insights into its function in chromatin dynamics and gene regulation.Understanding the role of HIST1H2BN in gene expression and chromatin organization is essential for unraveling the complexities of epigenetic regulation and its impact on human health. The HIST1H2BN Polyclonal Antibody is a reliable tool for investigating the functions of histone proteins and their implications in disease processes.
Antibody Name:
HIST1H2BN Antibody (PACO40210)
Antibody SKU:
PACO40210
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:100-1:1000
Species Reactivity:
Human
Immunogen:
Recombinant Human Histone H2B type 1-N protein (2-126AA)
Immunohistochemistry of paraffin-embedded human glioma using PACO40210 at dilution of 1:100.
IHC image of PACO40210 diluted at 1:600 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:
Histone H2B type 1-N (Histone H2B.d) (H2B/d), HIST1H2BN, H2BFD
UniProt Protein Function:
H2B1N: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.1Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: DNA bindingBiological Process: nucleosome assembly
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
