HIST1H2BL Antibody (PACO62203)
- SKU:
- PACO62203
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- IHC
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
HIST1H2BL Antibody (PACO62203)
The Hist1H2BL Polyclonal Antibody (PAC06203) is a valuable tool for researchers studying histones and chromatin structure. Hist1H2BL is a member of the histone H2B family, which plays a role in packaging DNA into chromatin and regulating gene expression. This antibody, raised in rabbits, is highly specific for human Hist1H2BL and is validated for use in various applications, including Western blot and immunohistochemistry.Histones are essential proteins that help organize and compact DNA in eukaryotic cells, playing a crucial role in gene regulation and genomic stability.
Dysregulation of histone proteins can lead to various diseases, including cancer and developmental disorders. The Hist1H2BL Polyclonal Antibody enables researchers to study the expression and localization of Hist1H2BL in different cell types and tissues, providing important insights into chromatin remodeling and gene transcription processes.
| Antibody Name: | HIST1H2BL Antibody (PACO62203) |
| Antibody SKU: | PACO62203 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, IHC |
| Recommended Dilutions: | ELISA:1:2000-1:10000, IHC:1:200-1:500 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Histone H2B type 1-L protein (2-126AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | IHC image of PACO62203 diluted at 1:300 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
 | IHC image of PACO62203 diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Synonyms: | Histone H2B type 1-L (Histone H2B.c) (H2B/c), HIST1H2BL, H2BFC |
| UniProt Protein Function: | H2B1L: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p22.1Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: DNA bindingBiological Process: nucleosome assembly |
| UniProt Protein Details: | |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | Q99880 |
| NCBI GenInfo Identifier: | 7387743 |
| NCBI Gene ID: | 8340 |
| NCBI Accession: | Q99880.3 |
| UniProt Secondary Accession: | Q99880,Q52LW9, B2R5A3 |
| UniProt Related Accession: | Q99880 |
| Molecular Weight: | 13,952 Da |
| NCBI Full Name: | Histone H2B type 1-L |
| NCBI Synonym Full Names: | histone cluster 1, H2bl |
| NCBI Official Symbol: | HIST1H2BLÂ Â |
| NCBI Official Synonym Symbols: | H2B/c; H2BFC; dJ97D16.4Â Â |
| NCBI Protein Information: | histone H2B type 1-L |
| UniProt Protein Name: | Histone H2B type 1-L |
| UniProt Synonym Protein Names: | Histone H2B.c; H2B/c |
| Protein Family: | Histone |
| UniProt Gene Name: | HIST1H2BLÂ Â |
| UniProt Entry Name: | H2B1L_HUMAN |
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