The Hist1H2BD Polyclonal Antibody (PAC045898) is a valuable tool for researchers studying histone H2B type 1-D (Hist1H2BD), a member of the histone family of proteins that play a key role in chromatin structure and gene expression regulation. This antibody, produced in rabbits, exhibits high specificity and sensitivity for human samples, making it an excellent choice for Western blotting applications.Histone H2B proteins, including Hist1H2BD, function as part of the nucleosome core particle, around which DNA is wrapped in the cell nucleus. They are involved in the packaging of DNA and the regulation of gene transcription processes.
Understanding the expression and modification of Hist1H2BD is essential for research in epigenetics, gene regulation, and chromatin dynamics.By utilizing the Hist1H2BD Polyclonal Antibody, researchers can explore the role of this histone variant in various cellular processes and diseases, including cancer, developmental disorders, and epigenetic regulation. Its high reactivity and specificity make it a valuable tool for investigating the complex connections between histone proteins and gene expression.
Immunohistochemistry of paraffin-embedded human ovarian cancer using PACO45898 at dilution of 1:100.
Immunofluorescent analysis of Hela cells using PACO45898 at dilution of 1: 100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Immunohistochemistry of paraffin-embedded human glioma using PACO45898 at dilution of 1:100.
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Synonyms:
Histone H2B type 1-D (HIRA-interacting protein 2) (Histone H2B.1 B) (Histone H2B.b) (H2B/b), HIST1H2BD, H2BFB HIRIP2
UniProt Protein Function:
H2B1D: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Interacts with acetylated H4 during chromatin remodeling. Binds to HIRA, a replication-dependent histone family member.Protein type: DNA-bindingChromosomal Location of Human Ortholog: 6p21.3Cellular Component: cytoplasm; nucleoplasm; nucleusMolecular Function: DNA bindingBiological Process: nucleosome assembly
UniProt Protein Details:
NCBI Summary:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015]
